Effect of diisopropyl 1,3-dithiolan-2-ylidenemalonate(NKK-100) on the components of hepatic microsomal mixed function oxidase in male broiler chicks.

Abstract

Effect of oral administration of diisopropyl 1, 3-dithiolan-2-ylidenemalonate (NKK-100) on the components of mixed function oxidase system in hepatic microsomes was investigated in male broiler chicks. Feeding NKK-100 increased relative liver weight and microsomal protein content of the liver. Contents of cytochrome P 450 and b 5 per mg microsomal protein and g liver increased with NKK-100 levels. The activities of NADPH-cytochrome c reductase and NADH-cytochrome b 5 reductase per g liver were enhanced by feeding NKK-100, while those activities per mg microsomal protein remained unchanged. Pretreatment with clopidol (an anticoccidial agent) and colistin (an antibiotic) did not affect the components of MFO. These results suggests that NKK-100 has a property to activate the hepatic microsomal MFO and the effect of NKK-100 are more prominent in the increases in specific activities of cytochrome P 450 and b 5 rather than in those of NADPH-cytochrome c reductase or NADH-cytochrome b 5 reductase.