Abstract
1. Diflubenzuron and polyoxin D clearly inhibited the incorporation of [3H]-N-acetylglucosamine ([3H]NAGA) into chitin in the isolated integument from newly molted American cockroaches under the experimental condition. 2. Upon homogenization, or tissue slicing, such an inhibitory effect of diflubenzuron on chitin synthesis totally disappeared, while that of polyoxin D did not. 3. Mitochondrial poisons (oligomycin and FCCP), protein phosphorylation modulations (8-Br-cAMP, Na3VO4 and MnCl2), potassium ionophores (valinomycin), and calmodulin inhibitor (trifluoperazine) clearly inhibited [3H]NAGA incorporation. 4. Phospholipase A2 and calcium ion significantly enhanced the [3H]NAGA incorporation.
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