Abstract
Zinc incorporation and the growth of Saccharomyces cerevisiae were investigated in a culture supplemented with three inorganic zinc salts, i.e. zinc sulphate, zinc chloride and zinc nitrate. The cultivation was performed on a yeast extract peptone dextrose (YEPD) broth medium. The growth of yeast was carried out at different concentrations of zinc, i.e. 0, 30, 60, 90 and 120mg 100ml-1. It was found that the addition of different zinc sources at 30mg 100ml-1 concentration produced higher biomass yield ranging 1.00-1.03g from 100ml-1 of cultivation medium, while higher zinc concentration in the medium caused significantly lower yields of yeast biomass. The amount of zinc in yeast cells was determined by an atomic absorption spectrometer (AAS). The highest amount of zinc in yeast cells was achieved when added in the form of zinc sulphate at a concentration of 120mg 100ml-1. The increment of intracellular zinc was up to 9889.67mgkg-1 of biomass.
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