Effect of different types of in vitro maturation medium (IVM) on maturation rate of non-vitrified and post vitrified-thawed pig follicular oocytes

Abstract

Purpose: To study the effect of different types of In vitro maturation medium on cumulus cell expansion and nuclear maturation rate of non-vitrified and post vitrified-thawed porcine follicular oocytes. Methods: Three different In vitro Maturation (IVM) media (IVM-I contained only hormones and growth factors, IVM-II contained porcine follicular fluid along with hormones and growth factors and IVM-III contained porcine follicular fluid, foetal bovine serum and growth factors without any hormone) were prepared using modified tissue culture medium (TCM-199) as a base medium for the study. Porcine oocytes were collected and IVM in above media was done for 48 h. Results: In case of non-vitrified and vitrified oocytes the rate of cumulus cells expansion did not differ significantly, but nuclear maturation rate differed significantly between IVM media. The nuclear maturation rate was significantly higher in IVM-II and IVM-III than in IVM-I both for non-vitrified and vitrified groups. Conclusion: Addition of follicular fluid to the IVM media leads to significantly higher nuclear maturation rate for both vitrified and non-vitrified porcine follicular oocytes.