Abstract
The present study was designed to determine the effect of different sugars supplemented to the extender on post-thaw quality of CLC treated ram sperm. The ejaculates (n=10) were divided into two aliquots: first aliquot was treated with CLC (3mg/120×106 sperm), whereas, second aliquot remained untreated and served as control. The samples were then incubated at 35°C for 15min. After incubation, each aliquot was again divided into 3 equal subgroups. Each of the subgroups was then diluted with one of the extenders containing fructose, sucrose or trehalose (28mM) to make a final sperm concentration 200×106/ml. After dilution, the semen samples were loaded into 0.25ml straws and cryopreserved using standard procedure. The data showed that type of the sugar did not affect post-thaw sperm parameters (P>0.05). However, the CLC pre-treatment improved (P<0.05) motile, live, live-intact, total intact and the number of total active sperm per straw. A non-significant interaction (P>0.05) was determined between type of sugar and CLC. In conclusion, CLC pre-treatment significantly improved post-thaw sperm parameters in ram and has no interaction with any of the used sugars.
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