Effect of different seed priming agents on chromium accumulation, oxidative defense, glyoxalase system and mineral nutrition in canola (Brassica napus L.) cultivars

Abstract

The present experiment was conducted to appraise the role of different seed priming agents in circumventing the negative impact of chromium (Cr) toxicity on canola plants. Chromium toxicity resulted in significant decline in photosynthetic pigments and growth attributes of two canola cultivars (Puriga and MS-007). Cr toxicity also resulted in higher oxidative stress mirrored as greater accumulation of hydrogen peroxide (H2O2) superoxide radical (O2•‒), electrolyte leakage (EL) and malondialdehyde (MDA). Further, lipoxygenase enzyme activity that catalyzes the peroxidation of membrane lipids was also enhanced due to Cr toxicity. Canola plants also manifested impaired methylglyoxal (MG) detoxification due to the downregulation of glyoxalase enzymes (GlyI and II) under Cr stress. Seed priming treatments viz. osmo-priming with calcium chloride (CaCl2) and hormonal priming with salicylic acid (SA) remarkably improved growth and chlorophyll content in both canola cultivars under Cr toxicity as compared to other priming treatments such as hydro-priming, redox priming (H2O2) and chemical priming (Se; selenium). Moreover, CaCl2 and SA seed priming also resulted in lower oxidative stress and improved enzymatic (SOD, POD, CAT, APX, GR, GST) and non-enzymatic (GSH, phenolics, flavonoids, proline) antioxidant system of both cultivars under Cr toxicity. Further, hormonal and osmo-priming strengthened glyoxalase and antioxidant systems, thus improving reactive oxygen species (ROS) and MG detoxification. In this background, the cultivar Puriga is considered Cr tolerant as it exhibited better growth and lesser oxidative stress in both seed priming and non-primed conditions under Cr toxicity than cv. MS-007.