Abstract

This research was conducted at Akdeniz University, Faculty of Agriculture's research greenhouse in 39 plastic pots. Different bacteria solutions (5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55 mL bacteria solution per 1 kg seed) were inoculated in the seeds of common vetch plant (Vicia sativa L.) with each replication. In addition, the most convenient bacteria dosage and differences between bacterial and artificial fertilizer applications were determined by applying (13 applications in total) sole fertilizer so that 1 kg of nitrogen (N) falls per decare in 1 pot and by applying control (non-inoculated seeds) for 1 pot. Analyses revealed that bacteria solution applications of 45 mL on plant height; 5 mL on plant nitrogen content; 0, 5, 10, 15 and 25 mL on plant phosphorus content; 25 mL on plant potassium content; 0, 5, 35, 40 and 45 mL on fresh weight; 55 mL on root weight; 30 mL on number of bacteria in soil; 30 mL on dry matter percentage and 5 mL on crude protein percentage have taken the first place. Fertilizer application did not sustain in any first place for any of the features, which indicate that bacteria applications can give better results.

Highlights

  • Leguminous plants compose a major part of plants used for animal feeding purposes all over the world

  • Inactive bacteria were dissolved in 0.5 mL Maximum Recovery Diluent (MRD), 0.9% sodium chloride, Tryptic Soy Broth (TSB), peptone water, sterile distilled water

  • Analyses revealed that application dosages of 45 ml on plant height; 5 ml on plant nitrogen content; 0, 5, 10, 15 and 25 ml on plant phosphorus content; 25 ml on plant potassium content; 0, 5, 35, 40 and 45 ml on fresh weight; 55 ml on root weight; 30 ml on number of bacteria in soil; 30 ml on dry matter percentage and 5 ml on crude protein percentage took the first place

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Summary

Introduction

Leguminous plants compose a major part of plants used for animal feeding purposes all over the world. They grow naturally in meadows and pastures and are essential in supplying roughage for animals. Vetches have bacteria called Rhizobium, which fix the free nitrogen in the air. If there isn't enough nitrogen in the soil, seedlings get damaged and their growth decelerate due to nitrogen deficiency. If these bacteria are not sufficient and effective in the soil, seeds should be inoculated with the appropriate type of Rhizobium according to their varieties (Önder and Akçin, 1991)

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