Abstract

Whey protein and essential amino acids are the most popular supplementation in sports nutrition. It has been recommended that protein plus carbohydrate in a certain ratio 1:3 or 1:4 improve the muscle glycogen synthesis and promote recovery. These benefits from protein and carbohydrate complex are compelling compared to supplement protein or carbohydrate along. However different protein ratio on blood glucose regulation remains unclear. PURPOSE: In this study we investigated a single supplement different protein beverage in different ratio on blood glucose regulation. METHODS: We used cross-over study design. Twelve heathy college baseball players (21±0 y). Thereafter the subjects were divided into four groups: protein0% (PRO0), protein12% (PRO12), protein36% (PRO36) and protein75% (PRO75). Fasting blood were collected. After drinking protein supplement, blood glucose and insulin were measured every 30 min for 2 h. The data were analysis by one-way ANOVA with repeated measures. RESULTS: PRO0 on glucose area under the curve (GAUC) was significantly higher than other groups (4241±446 vs. 2538±275, 1784±234, 196±287, P<0.05) and PRO75 GAUC was significantly lower than other groups (196±287 vs. 4241±446, 2538±275, 1783±234, P<0.05). After supplement 30 min PRO75 blood glucose was significantly lower than other groups (107±2 vs. 166±8, 139±6, 146±6mg/dL, P<0.05). After supplement 60 and 120 min PRO12 was significantly higher compare with PRO36 (123±3 vs. 107±4mg/dL, P<0.05 and 118±3 vs. 108±1mg/dL, P<0.05). PRO36 on insulin area under the curve (IAUC) was significantly higher compare with PRO75(2972±336 vs. 1178±158, P<0.05). After supplement 30 min PRO36 produced higher insulin concentration compare with other groups (56±7 vs. 31±5, 35±7, 14±2mU/L, P<0.05). CONCLUSION: High protein ratio did not have better glucose and insulin response. However PRO36 may increase insulin level faster than other groups, but did not secrete more insulin.

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