Abstract
Camellia euphlebia is a new food source and traditional folk medicine in China. Previous studies have demonstrated the antidepressant activity of Camellia euphlebia extract by both in vivo and in vitro experiments. The effects of different pretreatments on phytochemical contents and neuroprotective activity of Camellia euphlebia extract were further investigated in order to develop an optimal processing method that makes the extraction more efficient. Six different powders of Camellia euphlebia leaves were prepared by different pretreatments. The particle size and morphology were examined by using a Malvern particle size analyzer and scanning electron microscopy, respectively. The results showed that the percentage of powder particle size within a range of 0.2∼40 μm was up to 79.18% after press-shear assisted interaction technology pretreatment by 2% addition of shellfish shell powder, and the cells were broken completely. Additionally, the contents of flavonoids, polysaccharides, polyphenols, saponins, and catechin in the extract were 11.78 ± 0.62%, 34.60 ± 3.37%, 6.15 ± 0.29%, 9.43 ± 1.19%, and 1.99 ± 0.11%, respectively, which were higher than those of the other five extracts. Moreover, the extract had the strongest neuroprotective activity by comparing the neuroprotective effect of different extracts on corticosterone-induced neurotoxicity in differentiated PC12 cells. It is concluded that press-shear assisted interaction technology with 2% addition of shellfish shell powder pretreatment, to a great extent, improved the dissolution of bioactive ingredients in Camellia euphlebia.
Highlights
Our previous works found that Camellia euphlebia aqueous extract (CEE) exhibited an antidepressant-like activity in several animal behavioral tests including tail suspension test, forced swimming test, and chronic unpredictable mild stress test via the alteration of hypothalamic-pituitary-adrenal axis (HPA) and monoaminergic systems in vivo [2, 3] and protected against corticosterone-induced apoptosis in differentiated PC12 cells by regulating the mitochondrial apoptotic pathway and PKA/CREB/BDNF signaling pathway in vitro [4]
The results showed that the particle size of Camellia euphlebia powder prepared by Press-shear assisted interaction (PAI)-2 became smaller and more uniform
The results showed that the percentage of particle size of Camellia euphlebia leaves powder within a range of 0.2∼40 μm was up to 79.18% after PAI pretreatment with the addition of 2% shellfish shell powder, which significantly increased by 3.72 times and 1.28 times more than the 60 mesh powder and 200 mesh powder prepared by traditional methods, respectively (Table 1)
Summary
Our previous works found that Camellia euphlebia aqueous extract (CEE) exhibited an antidepressant-like activity in several animal behavioral tests including tail suspension test, forced swimming test, and chronic unpredictable mild stress test via the alteration of hypothalamic-pituitary-adrenal axis (HPA) and monoaminergic systems in vivo [2, 3] and protected against corticosterone-induced apoptosis in differentiated PC12 cells by regulating the mitochondrial apoptotic pathway and PKA/CREB/BDNF signaling pathway in vitro [4] These studies opened the possibility of Camellia euphlebia as an alternative dietary therapy to depression, the yield of CEE obtained from traditional processing methods is relatively low, directly hindering the development and utilization
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