Abstract
The effect of different pretreatment on callus induction from anther in three cultivars of balsam pear (Momordica charantia L.) was investigated. The results showed that cold pretreatment was the best for anther callus induction, and followed by heat shock, while centrifugation had no significant effect on callus induction from anther. Cold pretreatment for 24 h proved to be optimal as it significantly increased the callus induction rate, which varied with cultivars, reaching the maximum of 44.00, 51.10 and 72.67% for cv. Lvcui, Cuifei and Bixiu, respectively. Key words: Balsam pear (Momordica charantia L.), anther culture, callus induction, pretreatment.
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