Effect of different portal-azygos devascularization procedures on apoptosis of gastric mucosal cells in portal hypertension canines with cirrhosis

Abstract

Objective To detect apoptosis of gastric mucosal cells in portal hypertension canines with cirrhosis after different portal-azygos devascularization procedures and investigate their effects on portal hypertension gastropathy(PHG).Methods Portal hypertension was induced in canines by subcutaneous injection of 60% CCI4 emulsion and diet control.Twenty-four portal hypertension model canines were randomly divided 4 groups:group A(traditional paraesophagogastric devascularization group),group B (selective paraesophagogastric devascularization group),group C(selective paracsophagogastric devascularization plus distal esophageal transaction and reanastomosis with stapler group),and group D(control group,sham operation).The changes in free portal pressure(FPP)and apoptosis of gastric mucosa cells were observed during operation and 6 months after operation.Apeptosis index(AI)was measured by TUNEL.and the expression of Caspase-3 was detected by immunohistotehemistry.Results FPP in groups A.B and C were decreased significantly post-operation and at the 6th month post-operation as compared with pre-operation(P＜0.05).FPP in group A(2.05±0.07)kPa was significantly higher than that in groups B(1.28±0.05)kPa and C(1.41±0.04)kPa(P＜0.05),and that in group C hisher than in group B(P＜0.05).At the 6th month pest-operation,AI was increased significantly from(14.68±1.28)% to(20.22±2.20)% (P＜0.05),and the expression of Caspase-3 was also increased from 56.13±11.75 to 113.78±19.80(P＜0.05).In group B,AI was decreased from(13.63±1.19)% to (9.98±2.29)%(P＜0.05),and in group A the expression of Caspase-3 was decreased from 59.13±10.07 to 22.35±4.75(P＜0.05).In groups C and D,AI and the expression of Caspase-3 had no significant difference before and 6 six months post-operation(P＞0.05).Concision In portal hypertension canines with cirrhosis,the apoptosis of gastric mucosa cells is increased,and PHG aggravates after traditional paraesophagogastric devascularization.Selective paraesophagogastric devascularization can ameliorate the apoptosis of gastric mucosa cells and PHG.The apoptosis of gastric mucosa cells and PHG have no significant changes after selective paraesophagogastric devascularization plus distal esophageal transaction and reanastomosis with stapler. Key words: Portal hypertension; Devascularization; Stapler; Apoptosis; Canine