Effect of different pigmented cooked common beans on glucose and lipid metabolism in obese rats and 3T3 L1 cells

Abstract

The effect of Azufrasin (yellow coat seed) and Flor de Junio (FJ) Dalia (pink-cream coat seed) cooked bean varieties was evaluated in high fat and fructose diet-fed rats to identify their health beneficial effects on obesity-related metabolic alterations. FJ Dalia and Azufrasin daily supplementation (10%) for six months decreased body weight (5–7%), serum triglycerides (28.5–36.4%), HOMA insulin resistance index (59.6–62.3%), and serum TNF-α (40.5–45.0%); moreover, FJ Dalia increased serum adiponectin (77.5%). Polyphenol-, phytosterol-, and saponin-rich extracts from Azufrasin and FJ Dalia were evaluated in 3T3 L1 cells. Both varieties up-regulated, Cpt1a and Acadm (fatty acid β-oxidation), and Glut4, Irs1, and Pi3k (insulin signaling cascade) and down-regulated Pparg and Cebpa (adipogenesis) and Fasn and Acaca (lipogenesis). These activities were associated with their high content of soyasaponins A3, Af, and Be for adipogenesis and lipid metabolism, as well as eriodictyol, ellagic acid, hesperidin and (+)-catechin and (−)-epicatechin and their derivatives, and β-campesterol hexoside for insulin signaling cascade. FJ Dalia and Azufrasin cooked beans could be consumed to ameliorate obesity and its metabolic complications.