​​Effect of Different Media on in vitro Shoot Regeneration from Various Explants of Lilium longiflorum Cv. Elite, Brunello, Cordelia

Abstract

Background: Lilium (Lilium longiflorum Thunb.) belongs to the family Liliaceae and is a native of Northern Hemisphere (up to South Canada and Siberia). Conventionally, lilium can easily be propagated by sexual and asexual methods of propagation but these prevalent methods are not capable of meeting the increasing demand in domestic and global market. Generally, lilium is propagated through bulbs which has bottlenecks like high cost of planting material, infected from seed borne and other diseases like mosaic, grey mould, etc. So, in vitro multiplication of lilium is utmost important. Lily can be easily regenerated from explants The objective of present study was to identify the best media and the explant among scales and nodal segments belonging to the three different cultivars viz., Elite, Brunello, Cordelia where maximum number of shoots could be regenerated. Methods: The present investigation was carried out in the tissue Culture Laboratory of the Centre for Research and Application in Plant Tissue Culture. Twenty-five media combinations were used to study their effect on in vitro shoot regeneration from various explants of Lilium longiflorum cultivars. Two type of explants viz. scales and nodal segments were aseptically excised from Elite, Brunello, Cordelia cultivars of Lilum and cultured on Murashige and Skoog (MS) medium containing different concentrations and combinations of 6-benzyl amino purine (BAP), kinetin and Naphthalene Acetic Acid (NAA). Result: In cultivars Elite and Brunello, the maximum number of shoots were observed when nodal segments were used on media combination i.e., LM-8 (MS+BAP 3.0 mg/l+NAA0.1 mg/l). In cultivar Cordelia, scales showed the maximum response on media LM-24 (MS+Kinetin 2.0 mg/l+NAA 0.1 mg/l).