Abstract
A protocol for seed germination and seedling culture of Cymbidium aloifolium (L.) Sw. and C. bicolor Lindl. Was developed. Green “matured” capsules containing seeds were surface sterilized and cultured on various media compositions. Murashige and Skoog (MS) medium with or without 10% coconut water (CW) promoted higher rate of initial seed germination. This was followed by modified Knudson’s C and Phytamax media supplemented with or without 10% CW. Further studies on seedling multiplication revealed that MS medium supplemented with 10% CW was more effective in axillary shoot proliferation. Rooted seedlings were successfully acclimatized to the greenhouse environment. Results of this study demonstrate that Cymbidiums can be successfully propagated through asymbiotic germination method for possible use in re-introduction in situ and conservation projects.
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