Effect of different masticatory functional and mechanical demands on the structural adaptation of the mandibular alveolar bone in young growing rats

Abstract

The influence of masticatory functional and mechanical demands on the structural adaptation of the alveolar bone has not been investigated in both animals and humans. The effect of two experimental factors, the insertion of a bite-opening appliance and the alteration of food consistency, was investigated in young growing rats, with a particular emphasis on three-dimensional (3D) bone microstructure. Thirty-six male albino rats were divided into two equal groups, fed with either the standard hard diet or soft diet, at the age of 4 weeks. After 2 weeks, half of the animals in both groups had their upper molars fitted with an upper posterior bite block, an appliance similar to those used in clinical orthodontics. The remaining animals served as a control. After another 4 weeks, the animals were sacrificed, and their left hemimandibles were excised. Bone mineral density (BMD) and bone microstructure parameters of the alveolar process were subsequently measured, using dual-energy X-ray absorptiometry (DXA) and micro-computed tomography (micro-CT). The alveolar process width was also measured. Both experimental factors led to significant shape and structure modification of the mandibular alveolar bone in the growing rat. The bite block applied a continuous light force, which was associated with an inhibition of alveolar process vertical growth and a significant increase of cortical thickness. Soft diet and the consequent reduction of the intermittent forces applied to the alveolar bone during mastication resulted in a reduction of bone mineral density, accompanied by decreased trabecular bone volume and thickness. This rat model could prove to be a useful tool for the in vivo investigation of the role of muscular forces on the shape and structure adaptation of bone.