Effect of different levels of Moringa oleifera seed powder to the diet on some immunological and histological parameters of common carp fish Cyprinus carpio L.

Abstract

75 common carp fish with an average weight of 65.08±0.42 g were used. The fish were fed on experimental diets, which were divided into five equal treatments in terms of protein percentages, different in the proportions of adding Moringa seed powder, was 0, 0.5, 1, 1.5 and 2%, respectively; the fish were fed on the experimental diets at 5% of the live weight, divided into 4 meals a day. The liver histological examinations in T2 and T3-treated fish showed a significant improvement in the histological characteristics of hepatocytes and the contents of the liver tissue parenchyma. At the same time, it was observed that the liver tissue was slightly affected in the fish of the fourth and fifth treatments without affecting the health status of the fish. Histological examinations also showed the improvement and superiority of T2, T3, T4 and T5 treatment fish compared to control treatment fish on histological parameters of small intestine and gill tissue. This indicates that adding Moringa seed powder to fish diets at low rates stimulated the immune system and raised the concentration of IgM and IgG immunoglobulins to improve the fish's tissue health status. Keywords: Moringa oleifera, immunological, histological, common carp fish Cyprinus carpio L.