Abstract
Attempts to improve somatic embryogenesis of five pecan (Carya illinoinensis L.) cultivars using different levels of blue-green aIgal extract added to the media proved effective. WPM4-pecan media, containing 1.0 g malic hydrozide/liter and 30 g sucrose/liter, for embryo enlargement showed that the higher the extract concentration the longer the embryo harvested after 7 to 8 weeks of incubation at 22 to 25C. `Desirable' responded the best to the algal extract, where the percent elongation recorded was 129.1, 177.3, 174.2, and 200.6 for 0%, 1%, 2%, and 4%, respectively. Dessicating the embryos at 5C for 5 days enhanced the conversion on WPM4 conversion media containing silver nitrate and GA3. The number of normal shoots and roots was higher at 1% extract in cultivars Muhan, Elliot, and S-17, while the 4% algal extract was more effective for `Desirable' and `Shawnee'. Algal extract had no effect on media pH. Survival of converted embryos in the greenhouse was promising.
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