Effect of Different Factors During In Vitro Growth and Multiplication in Groundnut Cultivar ‘JL-24’

Abstract

During the present study effect of different factors were studied during in vitro growth and multiplication of adventitious shoot buds induced from de-embryonated cotyledon explants of groundnut cultivar ‘JL-24’. Optimum shoot multiplication rate of 7.0 folds and average shoot length of 3.0 cm was achieved on Murashige and Skoog’s (MS) medium supplemented with 2.0 mgl−1 6-benzylamino purine. During subsequent subculture after every 21 days, an initial shoot number of 3 shoots per explant produced a maximum of 21 shoots per explant after 9 weeks. Sucrose at 3.0 % concentration supported the optimum shoot multiplication rate of 7.0 folds. Lowering or increasing sucrose concentration from the control (3.0 %) proved detrimental for in vitro shoot multiplication and growth. In case of liquid MS medium (without agar) supplemented with 6-benzylamino purine (2.0 mgl−1) and sucrose (3.0 %) the multiplication rate was reduced by 1.5 folds (after 6 weeks) as compared to the semi-solid medium (with 0.8 % agar). Hyperhydricity was also observed in liquid medium. Shoots measuring 3.0–4.0 cm obtained from multiplying cultures were found suitable for rooting. Cent per cent rooting was achieved on 1/4th MS medium with sucrose (2.0 %), agar (0.8 %) and indoleacetic acid (0.5 mgl−1). Subsequent hardening and acclimatization led to 95 % survival of in vitro raised plants.