Abstract
The effects of different extenders and storage periods on the motility and fertilization rate of rainbow trout (Oncorhynchus mykiss) semen were evaluated after short-term storage. Semen was collected from anesthetized males by the abdominal massage. After determination of main semen characteristics, the pooled ejaculates were diluted with 4 different extenders at a ratio of 1:3, and stored at 4oC for 72 h. During preservation, spermatozoa motility (%) were determined every 12 h. Fertilization was carried out using the dry fertilization technique. The sperm-egg ratio was approximately 0.5x106 sperm/egg for optimum fertilization success. The highest motility (64.4±5.27%) and fertilization rate (94.3 ± 0.58 %) were obtained from semen stored with glucose based extender after 72 h storage. These results indicate that glucose based extender is a better preservative than the other solutions used in the study for the short term preservation of rainbow trout semen.
Highlights
Preservation of fish sperm for short-term duration is generally useful from the commercial point of view and facilitates various hatchery operations
Extender solutions can be mixed with semen to increase the volume of the semen samples and to prevent it from deteriorating while it is being held or shipped for insemination
The sperm concentration in the seminal plasma varied between individuals and ranged from 1.6 x 109 to 15.4 x 109 sperm cells per milliliter of semen
Summary
Preservation of fish sperm for short-term duration is generally useful from the commercial point of view and facilitates various hatchery operations. The short-term storage of sperm at low temperature (4oC) is mostly applied in short-distance transport of gametes collected in different locations, in synchronizing the timing of obtaining good quality of gamete collection from males and females during artificial insemination, in avoiding the aging of sperm, in facilitating hatchery operations, in experimental programs for genetic studies [1,2,3]. The fish sperm could be preserved by storage in undiluted and diluted form. Undiluted sperm stored at low temperature has been reported to cause a reduction in fertilization capacity [4]. Storage of diluted sperm with extender provides better control compared to undiluted storage [5]. Extender solutions can be mixed with semen to increase the volume of the semen samples and to prevent it from deteriorating while it is being held or shipped for insemination
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