Effect of Different Egg Yolk Concentration on Chilled Barbari Buck Semen During Short Term Storage

Abstract

The experiment was designed to evaluate the optimal concentration of egg yolk in extender for diluting Barbari buck semen during short-term storage at 4°C. A total of four Barbari bucks were used as semen donors during the experiment. The observed mean (±SE) valuesrecorded for percent live spermatozoa ranged from 63.5 ± 1.08 to 75.33 ± 0.99, percent progressive motile spermatozoa ranged from 54.17 ± 1.56 to 69.00 ± 0.82, percentage of HOST positive spermatozoa ranged from 60.83 ± 1.62 to 71.33 ± 1.54 while the acrosomal integrity ranged from 58.67 ± 1.563 to 71.33 ± 0.92 during the experiment. Three different patterns to evaluate capacitation like changes were observed under fluorescence. The observed mean (±SE) values of pattern F ranged from 45.67 ± 1.54 to 62.33 ± 1.14, pattern B ranged from 22.00 ± 0.73 to 28.83 ± 1.58 while pattern AR represent capacitated spermatozoa ranged from 15.67 ± 0.71 to 25.00 ± 0.62 during the experiment. A significantly higher value (p<0.01) of different seminal attributes was recorded in samples diluted with 15% egg yolk followed by 10%, 20%, 5% and 2.5%. The results recorded during the experiment indicates that 15% egg yolk in the semen extender is best suited for semen dilution during chilling process in Barbaribuck.