Effect of different durations of exercise on transferrin-bound iron uptake by rat erythroblast

Abstract

This study evaluated effects of different durations of exercise on transferrin receptor (TfR) expression on the membrane of rat erythroblasts. Female rats were assigned to six groups: 3, 6 and 12 months of strenuous exercise (swimming 2 h/day, 5 days/wk) groups and their corresponding controls. At the end of experiments, the erythroblasts were isolated for Tf binding assay and transferrin-bound iron (Tf-Fe) uptake. Tissue non-heme iron and hematological iron indices were also measured. The TfR number on the cells was about 603,189 ± 107,562, 890,150 ± 164,849 and 384,695 ± 46,295 molecules/cell in three control groups (3, 6, 12 months) respectively. Exercise groups had significantly higher levels of TfR than those of the control groups, being 1,374,137 ± 243,677, 2,175,360 ± 462,737 and 1,012,759 ± 249,423 molecules/cell in 3, 6 and 12 months of exercise groups respectively (p < 0.05). After 30 min of incubation, cellular Tf approached to levels of 8.28 ± 1.94, 10.73 ± 3.30 and 6.60 ± 0.93 fmole/10 6 cells in 3, 6 and 12 months of exercise groups, while the corresponding control values were 3.09 ± 0.36, 5.03 ± 1.01 and 2.51 ± 0.88 fmole/10 6 cells respectively (all P < 0.05). The rates of cellular iron accumulation were 7.07 (3), 8.79 (6) and 5.96 (12 month) fmole/10 6 cells/min in the exercised rats and 2.91, 3.85, and 2.03 fmole/10 6 cells/min in their corresponding controls (all p < 0.05). However, no significant difference was observed in the ratios (Exercise/Corresponding control) of the increased TfR expression, Tf-Fe uptake and Tf endocytosis as well as of the decreased plasma iron and tissue non-heme iron levels induced by different periods of exercise. Furthermore, the increase in the length of exercise (6 or 12 month) did not induce a remarkable decrease in plasma hemoglobin and hematocrit. These results indicate that a true iron deficiency or ‘sport anemia’ can not develop even if under longer periods (6 or 12 month) of strenuous exercise.