Effect of different doses of 17α-methyl testosterone (17-α MT) hormone on male broodstock of tiger shrimp Penaeus monodon sperm quality

Abstract

This study aimed to determine the optimum dose of 17α-MT hormone to improve male shrimp P. monodon sperm quality. Male tiger shrimp P. monodon originated from pond with an average weight (57.56 ± 12.79) g were transferred to hatchery and acclimated for 1 week prior treatments. Shrimps were set up in controlled tanks in 5 shrimps/tank density. Treatments were 17α-MT hormone induction in different doses, i.e. A = control (ablation); B = 200 ng/100 g of broodstock body weight (BW); C = 300 ng/100 g BW; and D = 400 ng/100 g BW. The 17-α MT hormone was given using injection method every 7 days in 3 times frequency. Research was completely randomized designed with 4 treatments and 2 replications. Observed variables were: the amount of shrimps which carried spermatophores, weight of spermatophores, quantity of spermatozoa, spermatophores histology and water quality. Data of the amount of shrimps which carried spermatophores, weight of spermatophores and the quantity of spermatozoa were analyzed using analysis of variance (ANOVA), while data of spermatophores histology and water quality were analyzed descriptively. The amount of shrimps which carried spermatophores and weight of spermatophores were not significantly different (P>0.05), but spermatozoa quantity was significantly different (P<0.05). The highest spermatozoa quantity was obtained at 300 ng/100 g BW dose in fourth gonad maturity stage. The 17α-MT in 300 ng/100 g BW was the optimum dose for P. monodon sperm quality improvement and it could be applied to replace ablation method.