Effect of different dietary β -glucan dosages on regulation of stress response in rainbow trout infected by Aeromonas salmonicida

Abstract

PDF HTML阅读 XML下载 导出引用 引用提醒 β-葡聚糖对杀鲑气单胞菌感染后虹鳟应激过程的调节作用 DOI: 作者: 作者单位: 1. 中国科学院海洋研究所, 山东 青岛 266071;2. 中国科学院大学, 北京 100039;3. 大连海洋大学 海洋科技与环境学院, 辽宁 大连 116023 作者简介: 纪利芹(1989-),女,博士,研究方向为鱼类营养与免疫.E-mail:goofyji@126.com 通讯作者: 中图分类号: S942 基金项目: 国家自然科学基金项目（41306152，31472312，31402283）；第56批中国博士后科学基金项目（2014M560580）；山东省博士后创新基金项目（201402005）；国家科技支撑计划项目（2014BAD08B09）；江苏省重点研发计划项目（BE2015325）；青岛市海洋经济创新发展示范城市重点项目。 Effect of different dietary β-glucan dosages on regulation of stress response in rainbow trout infected by Aeromonas salmonicida Author: Affiliation: 1. Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2. University of Chinese Academy of Sciences, Beijing 100039, China;3. School of Marine Science and Environment Engineering, Dalian Ocean University, Dalian 116023, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:为研究不同浓度的）应激过程的调节作用，在虹鳟饲料中添加不同浓度的-葡聚糖（0，0.05%，0.1%，和0.2%），投喂42 d后感染杀鲑气单胞菌，在感染后第2，4和6天取样检测血清应激指标的变化，第7天时统计虹鳟的存活率。结果表明，添加-葡聚糖组的血清总蛋白（total protein，TP）浓度显著高于对照组（<0.05）。0.1%和0.2%组的血清丙二醛（malondialdehyde，MDA）活性在感染后第2天达到峰值，早于0.05%和对照组。0.1%和0.2%组血清髓过氧化物酶（myeloperoxidase，MPO）活性在感染后显著上升，而0.05%和对照组却显著下降（P<0.05）。P<0.05）。感染后第4天，<0.05）。总胆红素（total bilirubin，T-BIL）浓度在感染后显著下降（<0.05），其中0.2%组下降幅度最小。各组肝脏和脾脏热休克蛋白70基因（heat shock protein 70，HSP70）表达量在感染后都显著升高（-葡聚糖能显著提高感染杀鲑气单胞菌的虹鳟的存活率和有效减弱细菌感染引起的机体应激反应，其中0.2%剂量的保护效果最好且对应激过程的减弱效果最明显（<0.05）。 Abstract:In recent years, immunostimulants have become a new research field in aquaculture. -glucan is one of the most important immunostimulants, which can enhance the body's resistance to infection by improving the non-specific and cellular immunity of animals. The present study evaluated the effects of different dietary -glucan dosages (0, 0.05%, 0.1%, and 0.2%) on the anti-infection ability of rainbow trout () after 42 days of feeding. Thereafter, rainbow trout were infected with and sampled on the 2nd, 4th, and 6th days post-infection. Survival rates as well as the regulatory processes of serum stress-related factors were analyzed. Survival rates in the -glucan groups increased significantly compared with those in the control group after P<0.05). On the 4th day after infection, the serum total protein concentrations in all -glucan groups were significantly higher than those in the control group (<0.05). Serum malondialdehyde activity in the 0.1% and 0.2% groups reached a peak on the 2nd day post-infection, which was earlier than in the other infected groups. The activity of serum myeloperoxidase in the 0.1% and 0.2% groups increased significantly after infection, whereas in the 0.05% and control groups, it decreased significantly (-glucan groups after infection was significantly lower than that in the control group (-glucan groups increased more obviously than those in the control group (<0.05). On the 4th day after infection, the serum urea nitrogen concentration in all -glucan groups was markedly lower than that in the infected control group (<0.05). Total bilirubin (T-BIL) concentration decreased significantly after infection; however, T-BIL in the 0.2% group was clearly higher than that in the other groups. After infection, expression in the liver and spleen in all groups increased significantly. In the 0.1% and 0.2% groups, it reached a higher peak earlier than in the control group. Overall, the liver and head kidney in rainbow trout were damaged to a certain extent after infection with -glucan can effectively protect fish from damage caused by -glucan showing the best effect. 参考文献 相似文献 引证文献