Abstract
Aim:This study aims to determine the effect of the stationary rawx, roller, and the suspension cell culture systems on the total virus yield infectivity and antigenicity.Materials and Methods:Three serotypes of foot and mouth disease virus (FMDV) (serotype A, O and SAT-2) were inoculated separately into baby hamster kidney-21 cell line in rawx, roller, and suspension cultivation systems using multiplicity of infection (1:100). Samples were taken from the total virus yield from each system at 15, 18, 21, and 24 h post-inoculation. Testing the total virus yield infectivity through virus titration and antigenicity through estimation of complement fixing titer and 146S content and evaluation of the potency of the vaccine prepared from the different cultivation systems were done.Results:The results showed that the FMDV titer of serotype A, O, and SAT-2 obtained from the roller cultivation system showed the highest level followed by suspension cultivation system then the rawx cultivation system. The FMDV titer showed its highest level at 21 h post-inoculation in all the cultivation systems and then decline at 24 h post-inoculation. The antigenicity reached its highest value content at 18 h post-inoculation either by complement fixation test or by quantifying the 146S intact virion. Montanide ISA 206 oil inactivated trivalent vaccines were prepared from the tested serotypes (A Iran O5. O Panasia and SAT-2/EGY/2012) harvested at 18 h post-inoculation from the 3 culture systems. The results of tracing the antibody response showed that the mean antibody response from the roller cultivation system start its protective antibody titer earlier at 2 weeks post-vaccination (WPV) than the vaccine prepared from the other two cultivation system and the immune protection period lasts longer for 36 WPV for the roller cultivation system vaccine than the other two cultivation systems.Conclusion:The best cultivation system used for the production of FMD vaccine regarding its highest infectivity and antigenicity is the roller system.
Highlights
Foot and mouth disease (FMD) is considered a highly infectious disease of cattle, sheep, goats, and pigs
The results showed that the foot and mouth disease virus (FMDV) titer of serotype A, O, and SAT-2 obtained from the roller cultivation system showed the highest level followed by suspension cultivation system the rawx cultivation system
As the baby hamster kidney (BHK)-21 cell line either the monolayer or the suspension systems are used in FMD vaccine production and to ensure the potency and the quality of the vaccine produced, It was of interest to follow-up the infectivity, complement fixing activity and 146S content of FMDV type A, O and SAT-2/Egypt 2012 on BHK cell culture in roller, rawx and suspension culture systems
Summary
Foot and mouth disease (FMD) is considered a highly infectious disease of cattle, sheep, goats, and pigs. It affects wild animals such as buffaloes and deer [1,2]. It is characterized by rise in body temperature with saliva drooling from its mouth due to vesicular lesions on the tongue, gums, cheeks, and hard palate. There is 100% morbidity in susceptible animal population and negligible mortality in adults. High mortality in a young animal is due to the virus-induced necrotic lesions and myocardial degeneration [3,4]
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