Abstract

This study aimed to evaluate the effect of cooking (boiling, steaming, and frying) on polyphenols, flavonoids, carotenoids and antioxidant activity of six edible leaves. The total antioxidant capacity of the fresh and cooked leaves was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and singlet oxygen scavenging assays. The results revealed that frying caused a reduction in major bioactives and antioxidant activities in all leafy vegetables tested. However, steamed and boiled leaves of C. auriculata and C. asiatica have shown greater levels of polyphenols, flavonoids, and antioxidant capacity compared with fresh leaves. Polyphenol and flavonoid contents of boiled S. grandiflora and G. lactiferum were higher than that of their fresh form. Boiled and steamed O. zeylanica and S. grandiflora have shown higher carotenoids. Boiled and steamed leaves of P. edulis have shown higher antioxidant activity. The impact of cooking on the changes in bioactive concentrations and antioxidant capacities are dependent on the species and the method of cooking.

Highlights

  • The presence and diversity of phytochemicals such as polyphenols, flavonoids, and carotenoids in vegetables are important factors for human health

  • Many epidemiological studies have shown that the diet rich in antioxidants play an essential role in disease prevention [1] and free radicals are known to be a significant contributor to many degenerative diseases such as cancer, cardiovascular diseases, and diabetes

  • C. auriculata leaves showed more than two times higher total polyphenol content in boiled and steamed leaves, compared with its raw form

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Summary

Introduction

The presence and diversity of phytochemicals such as polyphenols, flavonoids, and carotenoids in vegetables are important factors for human health. Dietary antioxidants protect against free radicals such as reactive oxygen species in the human body. The green leafy vegetables represent essential nutritional constituents in any balanced diet, and they contain a range of health-related phytochemicals [1]. Cassia auriculata, Gymnema lactiferum, Olax zeylanica, Sesbania granadiflora and Passiflora edulis are some of the edible leaves widely consumed as leafy vegetables in Sri Lanka and other tropical countries. These leafy vegetables possess strong antioxidative properties [1,3]. Most leafy vegetables can be consumed in their fresh form, and some of them

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