Abstract
An evaluation of the efficacy of different concentrations of the entomopathogenic fungus, Beauveria bassiana (Balsamo) Vuillemin against the corn leaf aphid, Rhopalosiphum maidis (Fitch) (Hemiptera: Aphididae) was carried out at Maejo University Biological Control Technology Learning Center (MJU-BCTLC) from August to October 2015. Ten concentrations of B. bassiana ranging from 1×10 1 to 1×10 10 spores/ml were used in the bioassay under the laboratory and greenhouse conditions. The laboratory bioassay revealed that all ten concentrations were found pathogenic to R. maidis . The mortality obtained ranged from 43.60% to 85.40%, highest at 85.40% of the concentration of 1×10 10 spores/ml and lowest at 43.60% of the concentration of 1×10 3 spores/ml, three days after treatment (DAT). However, the percent mortality values were not significantly different. They decreased with decreasing spore concentrations. Under the greenhouse condition the aphid infestation was at 65 days after sowing (DAS). At the concentrations of 1×10 10 , 1×10 9 , 1×10 8 , 1×10 7 , 1×10 6 spores/ml. The aphid population (5.6, 7.0, 9.8, 11.0 and 19.2 aphids per plant) at 65 DAS were drastically reduced at 72 DAS to 1.2, 3.0, 5.0, 9.4 and 11.2 respectively. In the control and at the concentration of 1×10 3 , 1×10 2 and 1×10 1 spores/ml, the mean number of aphid population gradually increased at weekly interval starting from 58 DAS until the end of cropping period. The study showed that the concentration at 1×10 10 spores/ml was most effective to reduce the corn leaf aphid population.
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