Effect of Different Concentrations of BAP and Putrescine on Potato Microtuberization (cv. Agria)

Abstract

One of the most critical stages of produced virus free potato plants is rapid and efficient in vitro microtuberization. Plant growth regulators and their concentration is important factor on potato microtuberization. This study was conducted to develop a protocol for rapid microtuber formation for potato micropropagation by different concentrations of 6-Benzylaminopurine (BAP) (0, 1 and 2 mg.l-1) and five concentrations of Putrescine (0, 20, 40, 80 and 160 mg.l-1) with 8% sucrose and 8 g.l-1 agar. Present study was conducted as a factorial experiment based on completely randomized design with three replications in tissue culture laboratory to investigate the effect of different BAP and Putrescine concentrations. Lateral buds of cv. Agria from in vitro shoots were cultured as explants. Cultures were kept in germinator with constant darkness and temperature of 17±2°C. Variance analysis showed that none of the traits were affected by different concentrations of the Putrescine other than weight of the shoots. Also, the interaction effect of PUT×BAP on shoot weight was significant. However, the effect of different levels of BAP was significantly associated with the most trait of microtuberization. In this experiment, the best concentration of BAP for microtuberization was 1 mg.l-1. Also, the appropriate Putrescine level for increasing of microtuberization number and percentage was 80 mg.l-1 but the effect of Putrescine on microtuberization number and percentage was non-significant.