Abstract
Pikeperch, Sander lucioperca (L.), has been identified as one of the most perspective candidates for diversification of freshwater aquaculture. However, some aspects of production are still being developed, and controlled reproduction is one of the bottlenecks. The aim of the present study was to compare the effectiveness of different commercial spawning agents in the induction of final oocyte maturation (FOM) and ovulation in wild spawners. Within the study, four spawning agents [human chorionic gonadotropin (hCG), mixed human and horse gonadotropin (PG-600), carp pituitary (CPH) and mammalian GnRH analogue combined with metoclopramide (Ovopel)] in different thermal regimes (13 and 15 °C) were tested. In both thermal regimes, the highest (P < 0.05) ovulation rate among the treatment groups was obtained after stimulation with hCG (100 % in both cases). Latency time was the shortest in groups where CPH was used (2–3 and 3–4 days for 15 and 13 °C) and was similar in the remaining groups (3–4 and 4–5 days for 15 and 13 °C, respectively). Embryo survival was the highest in groups treated with hCG (78.9 and 81.3 % at hatching stage for 15 and 13 °C, respectively). Hormonal stimulation did not significantly affect spermiation rate or spermatozoa motility (P > 0.05). Based on the obtained results, hCG can be recommended for induction of FOM and ovulation in pikeperch. In addition, the thermal regime within the tested range seemed to have no effect on the reproduction outcome, and the application of lower temperature only prolonged the time of ovulation.
Highlights
The pikeperch, Sander lucioperca (L.), is a species of great economic importance on the European market
Four spawning agents [human chorionic gonadotropin, mixed human and horse gonadotropin (PG-600), carp pituitary (CPH) and mammalian gonadotropin-releasing hormone (GnRH) analogue combined with metoclopramide (Ovopel)] in different thermal regimes (13 and 15 °C) were tested
Two females died shortly after the eggs stripping (Tables 1, 2). The results of this experiment confirm previous reports on the shorter period of latency following the application of carp pituitary homogenate (CPH) as compared to Ovopel under each of the thermal regimes tested
Summary
The pikeperch, Sander lucioperca (L.), is a species of great economic importance on the European market. In recent years it has become an important species for diversification of freshwater aquaculture (Kestemont and Melard 2000; Philipsen 2008; Wang et al 2009a) This has necessitated developing procedures for intensive culture in closed recirculating systems (Kestemont et al 2007; Philipsen 2008). Artificial reproduction directly affects the effectiveness of further culture procedures and is one of the most important stages in intensive aquacultural production (Kucharczyk et al 2007; Muller-Bellecke and Zienert 2008; Wang et al 2009b; Zakesand Demska-Zakes 2009). Reproduction of freshwater fish from wild populations is often impossible without hormonal stimulation (Krejszeff et al 2009, 2010a) This does not apply to percids, in which ovulation has been observed without the need to use hormonal preparations (Kucharczyk et al 1996, 1998, 2007; Ronyai and Lengyel 2010). It is very important to verify the latency time for these species due to the application of different reproduction procedures, including the use of hormonal preparations
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