Effect of Different Combinations of Red and Blue LED Light on Growth Characteristics and Pigment Content of In Vitro Tomato Plantlets

Most Tahera Naznin,Cheol Ho Park,Md Obyedul Kalam Azad,Mark Lefsrud

doi:10.3390/agriculture9090196

Abstract

The aim of this study was to evaluate the growth characteristics and pigment content of tomato plantlets grown under various ratios of red (R) (661 nm) and blue (B) (449 nm) LED light. In this study, three different ratios of R and B (RB) light such as 5:01, 10:01, and 19:01 along with R (100%) were used. The photosynthetic photon flux density (PPFD), and photoperiod of the growth chamber was 120 ± 5 μmol m−2s−1 and 16/8 h (day/night), respectively. Tomato plantlets were cultured for six weeks in the growth chamber. It was shown that tomato plantlets had higher photosynthesis rate, higher pigments content, higher growth characteristics (e.g., number of leaves, leaf area, shoot number, root number, root length, dry, and fresh mass), and greater surviving rate under the R:B = 10:01 ratio among the treatments. The plantlets showed at least a threefold decrease in photosynthesis rate, as well as a significant abnormal stem elongation when grown under 100% R light. It is concluded that the RB ratio of 10:01 showed excellent performance in all growth parameters. This result has shown that the optimum lighting environment improves tomato plantlet cultures in vitro.

Highlights

In vitro plant culture is a valued method in achieving improved disease free identical plant seedlings
It was observed that healthy healthy and vigorous tomato plantlets were attained when grown under the R and B (RB) ratio of 10:01 among and vigorous tomato plantlets were attained when grown under the RB ratio of 10:01 among the light the light treatments
The survival rate of tomato plantlets was higher when cultured under RB light

Summary

Introduction

In vitro plant culture is a valued method in achieving improved disease free identical plant seedlings. It has enormous roles in the propagation of plants in large quantities with desired characters [1]. Light is the most vital element to regulate in vitro plant growth and development. Artificial light should provide photons in the spectral region that is involved in photosynthesis and in the photomorphogenic responses of plant culture in vitro [2,3]. The development of in vitro plantlets can be improved by tuning the light quality, quantity, and photoperiod in the growth environment [5]

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Conclusion