Effect of different carbon sources on the in vitro multiplication of Annona sp.

José Raniere Ferreira De Santana,Renato Paiva,Lenaldo Muniz De Oliveira,Ana Valéria De Souza

doi:10.1590/s1413-70542011005000002

José Raniere Ferreira De Santana, Renato Paiva + Show 2 more

Open Access

https://doi.org/10.1590/s1413-70542011005000002

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Abstract

The Annonaceae family comprises approximately 2.300 species, some with significant commercial value. Although commercial plantations have suffered due to problems related to seedling production. As micropropagation is a viable technique for seedling production, the present work evaluated the effects of different carbon sources on in vitro bud induction in five Annonaceae species. Nodal segments obtained from plants of the Annona glabra, A. cauliflora, A. coriacea, A. bahiensis and Rollinia silvatica species were inoculated into solid WPM culture medium with 8.87 μM BAP, 0.86 mM of benomyl, and 87.64 mM of the following carbon sources: glucose, sucrose, fructose, galactose, sorbitol and maltose. We evaluated the buds number, the length and weight of the largest bud, the number of expanded leaves per bud, the length of the largest leaf and the dry matter of the buds. No significant difference was observed among the different carbon sources used in terms of the number of produced buds; however, the length of the largest bud, the number of expanded leaves, the length of the largest leaf, and dry weight of the buds presented significant difference according to the studied speciesas well as the carbon sources used, with the lowest value being obtained with sorbitol. The results obtained here indicated that, except for sorbitol, any of the carbohydrates tested could be used in the in vitro multiplication protocols for A. bahiensis, A. cauliflora, A. coriacea, A. glabra and R. silvatica.

Highlights

The family Annonaceae comprises approximately 132 genera and more than 2.300 woody species; approximately 50 species have edible fruits and some of them have commercial value
According to São José et al (1997) and Manica (2003), the most important species of Annonaceae cultivated in tropical regions are the “pinha” (Annona squamosa L.) and “graviola” (A. muricata L.), followed by “cherimoya” (A. cherimola Mill.) and “atemóia”
Nodal segments of A. bahiensis, A. cauliflora, A. coriacea, A. glabra, and R. silvatica were inoculated into test tubes (25x150 mm) containing 10 mL of WPM culture medium (Lloyd & McCown, 1980) solidified with 7 g L-1 of agar, and supplemented with 8.87 mM of BAP, 0.86 mM of benomyl, and 87.64 mM of one of the following carbon sources: glucose, sucrose, fructose, galactose, sorbitol, or maltose

Summary

INTRODUCTION

The family Annonaceae comprises approximately 132 genera and more than 2.300 woody species; approximately 50 species have edible fruits and some of them have commercial value. According to São José et al (1997) and Manica (2003), the most important species of Annonaceae cultivated in tropical regions are the “pinha” (Annona squamosa L.) and “graviola” (A. muricata L.), followed by “cherimoya” (A. cherimola Mill.) and “atemóia” (a hybrid between “pinha” and “cherimoya”) Other species, such as the “araticuns” (A. glabra, A. bahiensis and Rollinia silvatica), “marolo” (A. cauliflora) and “conde” (A. coriacea) have considerable commercial potential. Considering the importance of Annonas species within the context of fruit cultivation in Brazil and the necessity of determining the best techniques for seedling production at commercial scales, the present work evaluated the effects of different carbon sources on in vitro bud induction in five species of Annonaceae

MATERIAL AND METHODS

RESULTS AND DISCUSSION

CONCLUSIONS