Abstract
a-Galactosidase activity from Thermomyces lanuginosus strain of CBS 395.62/b was investigated in cultivation media with various compositions. Among the seven nitrogen sources only three L-asparagine, yeast extract and ammonium acetate supported the a-galactosidase synthesis. Ammonium acetate proved to be the best candidate as nitrogen source. When glucose or galactose was used as main carbon source, very low level, constitutive a-galactosidase activity was observed. In presence of raffinose, considerable a-galactosidase activity was detected. Raffinose can be replaced by sucrose in the cultivation medium, because the productivity reached by it was superior to that of raffinose. a-Galactosidase activity was improved by the optimisation of the concentrations of sucrose and ammonium acetate in the medium. Applying medium composition with 3% (w/v) sucrose and 0.6% (w/v) ammonium acetate led to at least 5 times higher activity which was observed in the reference medium containing 1.5% (w/v) raffinose and 0.45%...
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
