Abstract
Barringtonia racemosa L. has many medicinal properties especially the fruit and leaf parts. The fruits are used to relief pain and inflammation, the leaves were proved to control high blood pressure whereas the roots barks are effective to treat chicken pox. Due to its medicinal importance for human health, it is essential to conserve this plant. A comparative study of different types of media was performed to study its effect on callus growth of endosperm explant from B. racemosa. Types of basal media studied including Murashige and Skoog (MS), Lloyd and McCown Woody Plant (WPM) and Gamborg (B5). The endosperm explant of B. racemosa were cultured on different basal MS, WPM and B5 media supplemented with 1.0 mg/L 2,4-D and 1.5 mg/L kinetin. The observation of callus growth and morphological characteristics of callus were done on weekly basis within 4 weeks. After 4 weeks of incubation period, the maximum fresh weight (0.300 ± 0.027g) and dry weight (0.025 ± 0.003g) were recorded from the explants cultured on MS medium followed by WPM and B5 media supplemented with 1.0 mg/L 2,4-D and 1.5 mg/L kinetin. The textures of callus produced from MS were nodular in shape and creamy colour. In conclusion, all the media studied had successfully induced the callus growth of B. racemosa with the present of 1.0 mg/L 2,4-D and 1.5 mg/L kinetin.
Highlights
People had been used various materials from nature as beneficial sources to cure their ailment and maintain a good health
The highest callus induction percentage (100%) was found in the explants cultured on both Murashige and Skoog (MS) and Lloyd and McCown Woody Plant (WPM) media followed by Gamborg (B5) medium (85%) supplemented with 1.0 mg/L 2,4-D and 1.5 mg/L kinetin (Table 2)
Plant growth regulator 2,4-D and kinetin play an important role in callus induction since no callus was formed when the endosperm explants of B. racemosa were placed on Murashige and Skoog (MS), Lloyd and McCown Woody Plant (WPM) and Gamborg (B5) media without these plant growth regulators
Summary
People had been used various materials from nature as beneficial sources to cure their ailment and maintain a good health. One of the most usable sources from nature are plant, and as a result, large amount of modern drugs had been isolated and processed from various types of plant These types of plant that contributes to medicinal properties is known as medicinal plant. Barringtonia racemosa (L.) is a type of woody plant that possesses many benefits for maintaining human health such as lowering the blood pressure whereas and to treat the chicken pox [2]. Besides that, this plant contains high phenolic content with the presence of diterpines, triterpenoids, steroids and saponins can enhance the antioxidant activities [3]. Pharmacological activities of this species including antibacterial, anti-tumor, anti-nociceptive, antioxidant, anti-inflammatory, alpha-glucosidase inhibitor, antifungal, anti-tuberculosis, anti-arthritic and anti-diarrhoeal towards different parts of Barringtonia racemosa species were proven based on the previous study [5]
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