EFFECT OF DIETHYL SULPHATE ON M1 ATTRIBUTES AND SDS-PAGE ANALYSIS IN GROUNDNUT (Arachis hypogaea L.)

Abstract

The present study was carried out to know the effect of diethyl sulphate on groundnut cultivar TMV-7 particularly in M1 generation. The healthy and viable seeds of groundnut cultivar TMV-7 were exposed to different concentrations of diethyl sulphate (5 to 25 mM) and sown in the field. The various growth and morphological parameters like percentage of seed germination, survival percentage plant height, days to flowering, total height at time of harvest, root length and number of lateral roots, number of pods per plant and pod weight were decreased with increasing concentrations of diethyl sulphate. All the characteristics studied were inhibited when compared to control. The lower concentrations such as 5, 10 and 15 mM concentrations enhanced all the parameters. But there was a sudden decrease in 20 and 25 mM. The various concentrations of DES played a different role in the field condition itself. The shoots developed from different concentrations of DES showed variation in their number and height. The protein content also showed decreasing trend with increasing concentrations of DES. There was a steady increase of protein content up to 15 mM DES. After that, there was a drastic reduction in the protein content in 20 and 25 mM. From the diethyl sulphate treated seeds, the embryo axes were collected and subjected to SDS-PAGE analysis. The protein banding pattern also differentiated among various concentrations of DES. The protein band with 77.5 kDa was observed in 5 and 10mM treatments. Like that the 13.5 kDa was present in 10 and 25mM treatments. The 48.25 kDa recorded in 15 and 20 mM treatments.