Abstract
Simple SummaryDietary methionine is closely related to oxidative stress metabolism. Lambs were treated with methionine restriction and subsequent methionine re-feeding. The relationship among dietary methionine pattern, methionine metabolism, and antioxidant reactions was studied by detecting methionine metabolite content and antioxidant expression in order to provide theoretical basis for amino acid nutrition and healthy feeding of lambs.Our objective was to investigate the effect of methionine restriction and resuming supply on liver antioxidant response in lambs. The concentrations of methionine and its metabolites and the expression of the nuclear factor erythroid 2-related factor 2 (Nrf2), a redox sensitive factor, were detected after methionine restriction treatment for 50 days and methionine supply recovery for 29 days. The expression of glutathione (GSH) S-transferase (GST), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) were characterized at the level of transcription and translation. Methionine restriction can directly change the content of methionine and its metabolites in plasma and liver, and affect the redox state of lambs by activating the Nrf2 signaling pathway. Liver tissue can adapt to oxidative environment by upregulating the expression of antioxidant enzymes such as GSH-Px and SOD. Moreover, it was found that there was a lag effect in the recovery of metabolism after methionine supplementation.
Highlights
Published: 23 December 2020Accumulating evidence suggests that nutrition exerts considerable effects on health, and one of the main ways to improve health is through dietary interventions [1,2]
During the methionine restriction period, the intake of milk replacer and starter showed no difference between the two groups (p > 0.05)
This study demonstrated that methionine restriction affected the redox state of lambs
Summary
Published: 23 December 2020Accumulating evidence suggests that nutrition exerts considerable effects on health, and one of the main ways to improve health is through dietary interventions [1,2]. In the past few years, a core aspect of anti-aging research has been anti-aging strategies, which include time-restricted feeding, calorie restriction, fasting mimicking diets, and short-/longterm fasting. These strategies are conducive to optimizing the nutritional balance, preventing or alleviating a variety of diseases, such as metabolic disturbance, cardiovascular diseases, and autoimmune diseases [3,4,5]. Evidence from animal model studies shows that ROS production and oxidative damage can be reduced by dietary caloric or protein restriction, of which the Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
