Effect of dietary lipid level on growth, feed utilization and body composition by juvenile grass carp (Ctenopharyngodon idella)

Abstract

A study was undertaken to determine the effect of dietary lipid level on growth, feed efficiency and body chemical composition of juvenile grass carp. Seven isonitrogenous diets (400 g kg(-1) crude protein) containing seven dietary lipid level (0, 20, 40, 60, 80, 100 and 120 g kg(-1) dry matter) were fed to triplicate groups of 40 fish with initial weight 6.52 g, for 70 days. No obvious and assured essential fatty acid deficiency symptom appeared in fish fed the lipid-free diet. Excess dietary lipid level (100 and 120 g kg(-1)) resulted in decreased feed intake. The best growth performance and feed utilization was observed in fish fed 20-40 g kg(-1) dietary lipid. The fish fed a lipid-free diet had the lowest protein efficiency and protein retention. Growth performance and feed utilization increased with the increasing dietary lipid levels up to 40 g kg(-1) dietary lipid. Higher dietary level (above 40 g kg(-1)) made growth performance and feed utilization decrease and no protein sparing effect was observed. Lipid retention decreased as dietary lipid level increased. Mesenteric fat index (MFI) increased, hepatosomatic index (HSI) decreased with dietary lipid level. The increased MFI and simultaneous decrease lipid retention can be explained by differences in growth. The effect of dietary lipid levels on the chemical composition of tissues was significant only for whole body and muscle. The excess lipid content of liver in all groups was regarded as a slight symptom of fatty liver, which was partly identified by microscopic structural study and lower plasma lipid indexes, comparing to the initial plasma data. In conclusion, grass carp is a fish with low energy requirement and excess dietary lipid level should be avoided.