Abstract
Flavonoids are a class of compounds found in plants such as parsley, cocoa, soybeans, tea, and grapes. Flavonoids have anti‐oxidant properties, can act as retroviral inhibitors, and have cardiovascular benefits. We hypothesize that the cardiovascular benefits are, in part, due to an effect on salt and water homeostasis via the renal epithelial Na+ channel (ENaC). Quercetin, apigenin, and genistein are the dietary flavonoids of interest. Insulin and aldosterone regulate ENaC through a PI3‐kinase dependant pathway. A PI3‐kinase inhibitor, LY294002, was designed from the quercetin structure. Therefore, we hypothesize that flavonoids may inhibit ENaC activity. We used electrophysiological techniques to study the effects of flavonoids on ion transport in the mouse principle cells of the kidney cortical collecting duct (mpkCCD) cell line. All three flavonoids lower basal ion transport in mpkCDD cells. Neither genistein nor apigenin inhibit insulin stimulated ion transport. Following ADH stimulation, both flavonoids inhibit anion secretion via CFTR, but have no statistical effect on ADH‐stimulated Na+ reabsorption. In summary, in mpkCCD cells, the flavonoids lower basal, amiloride sensitive Na+ transport, but do not alter peptide‐hormone stimulated Na+ reabsorption. The flavonoids do, however, inhibit ADH‐stimulated anion secretion.Funding: IUPUI Undergraduate Research Opportunities Program.
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