Effect of dietary fish oil with or without vitamin E supplementation on fresh and cryopreserved ovine sperm

Abstract

This study was carried out to determine whether dietary fish oil (FO) with or without vitamin E (VE) supplementation would have a beneficial effect on characteristics of fresh and cryopreserved ovine sperm. Sixteen rams were allotted to four groups and fed one of the four diets: control diet (CON; without FO and VE supplementation), VE diet supplying 200 IU VE/day.ram, FO diet containing 2.5% (dry matter basis) FO, and OVE diet containing FO and VE. Semen samples were collected at Weeks 7, 9 and 11 for evaluation of seminal quality. Frozen samples from Weeks 9 and 11 were subjected to computer-assisted semen analysis (CASA). Seminal volume, percentage of sperm with normal morphology and intact acrosome and seminal concentration of malondialdehyde were increased in FO rams (P < 0.05). In VE rams, semen volume and percentage of morphologically normal sperm were higher compared with CON rams; however, in OVE rams these attributes were not different from those in CON rams. Other traits in fresh semen were not affected by the diet. Fish oil alone negatively affected the sperm viability, but not motility, of the frozen–thawed semen; however, simultaneous supplementation with FO and VE resulted in increased percentage of morphologically normal sperm, motility, and viability. The percentages of CASA type A (showing rapid progressive motility) and type B (showing medium progressive motility) sperm were higher in OVE compared with other groups. Vitamin E supplementation decreased the beat-cross frequency values compared with other groups. Other CASA parameters were not affected by the diets. In conclusion, dietary FO and VE in the breeding season of ram, generally, do not cause significant changes in fresh sperm quality but, for cryopreservation of sperm, supplementation of the diet with an antioxidant such as VE may be beneficial when polyunsaturated fatty acids are added to the diet.