Effect of dietary fish oil replacement by rapeseed oil on the growth, fatty acid composition and serum non-specific immunity response of fingerling black carp, Mylopharyngodon piceus

Abstract

An 8-week experiment on fingerling black carp Mylopharyngodon piceus was conducted to evaluate the effects of dietary fish oil (FO) supplement on growth, fatty acid composition and non-specific immunity responses. Five triplicate fingerling groups (initial weight = 2.72 ± 0.35 g) were fed isoenergetic and isonitrogenous diets in which the dietary FO was replaced with rapeseed oil (RO) in graded increments of 25% (0–100%). No significant effects were observed on specific growth rates, survival rates and feed conversion ratios, but there were significant differences in whole body moisture and liver lipid contents (P < 0.05), and the 100% RO replacement diet significantly enhanced hepatosomatic indexes compared to control group (P < 0.05). Other approximate whole body constituents, viscerasomatic ratios and condition factors were not influenced by dietary oil treatments. Fatty acid composition of muscle and liver was influenced by dietary fatty acid input, α-linoleic acid and γ-linolenic acid were significantly increased with increasing RO, but eicosapentaenoic acid, docosahexaenoic acid and the n-3/n-6 ratio were significantly reduced (P < 0.05). Alternative complement pathway, lysozyme and superoxide dismutase activities were not significantly influenced. These results indicate that black carp fed diets with FO supplement had similar growth and non-specific immunity to the fish fed diet with RO.