Effect of dietary cholesterol on the lipoprotein profile and binding of radioiodinated lipoproteins to hepatic membranes in the cockerel ( Gallus domesticus)

Abstract

1. 1. Cockerels feda cholesterol-supplemented diet experienced a marked elevation of lipoprotein particles of density ≤ 1.006 g/ml (VLDL) and a diminution of lipoprotein particles of density 1.02–1.05 g/ml (LDL). 2. 2. Unlike VLDL of some cholesterol-fed animals, cholesterol-fed cockerel VLDL did not display β-mobility on agarose gel electrophoresis. 3. 3. [ 125I]LDL and [ 125I]HDL binding to cockerel liver membranes was not affected by cholesterol feeding. 4. 4. Different lipoprotein types appear to bind to a common site on cockerel liver membranes. 5. 5. The results suggest that liver cells of cockerels may not possess LDL binding sites tat are analogous to those of mammalian species.