Effect of dietary chitosan on growth performance, hematological parameters, intestinal histology and stress resistance of Caspian kutum (Rutilus frisii kutum Kamenskii, 1901) fingerlings.

Abstract

This study was conducted to investigate the effects of dietary chitosan on growth performance, hematological parameters, intestinal histology, stress resistance and body composition in the Caspian kutum (Rutilus frisii kutum, Kamenskii, 1901) fingerlings. Fish (1.7±0.15g) were fed diets containing chitosan at different levels (0, 0.25, 0.5, 1 and 2gkg(-1) diet) for a period of 60days. Results showed that the feed conversion ratio significantly decreased in fish fed diet containing 1gkg(-1) of chitosan compared to the other groups (P<0.05), but there were no significant differences between treatments in terms of specific growth rate and condition factor (P>0.05). Leukocyte increased in fish fed diet containing 2gkg(-1) of chitosan compared to the other groups (P<0.05). Lymphocytes, eosinophils and neutrophils did not significantly change among dietary treatments (P>0.05). Also, the chitosan supplementation did not affect the whole-fish body composition (P>0.05). Light microscopy demonstrated that the intestinal villus length increased in fish fed diet containing 1gkg(-1) of chitosan compared to control group (P<0.05). While 11 and 13‰ salinity and 30°C thermal stress had no effect, 1gkg(-1) of chitosan (P<0.05) showed highest survival rate (70%) in 34°C thermal stress. The results showed that chitosan in the diet of the Caspian kutum fingerlings could improve feed conversion ratio, the nonspecific defense mechanisms and resistance to some of the environmental stresses.