Abstract
A technique for the extraction, purification and concentration of folates, followed by high performance liquid chromatography assay with fluorometric detection of the three principal derivatives (tetrahydrofolic, 5-methyl-tetrahydrofolic and formyl-tetrahydrofolic acids) has been applied to the determination of tissue folates in rats. The levels found are compared to those of the microbiological assay using Lactobacillus casei. When rats were fed a diet containing 1 mg of folic acid per kg of food, levels in the intestinal mucosae, liver, whole blood and brain were 0.59, 14.87, 0.28 and 0.83 nmol/g of tissue. An exogenous supply of 200 mg of folic acid/kg of food significantly increased folate levels in all tissues studied, except for the brain: 1.51, 28.93, 0.52 and 0.99 nmol/g, respectively in the above four tissues. The separation of the various derivatives and a variable supply of folic acid have enabled the conversions of these metabolites to be studied.
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