Abstract
To examine the manner in which dichloromethane inhibits sickling, sickle blood was subjected to both prevention and reversal schemes over a range of CH 2Cl 2 vapor pressures. Following CH 2Cl 2-treatment, the rotating frame spin lattice relaxation time (T 1ϱ) of water protons in deoxygenated packed sickle cells was measured, cell types in a deoxygenated fixed sample were counted, and the extent of hemolysis determined. At CH 2Cl 2 vapor pressures above 200 mm, the NMR relaxation rate decreased sharply, the extent of hemolysis increased, the fraction of sickled cells and other abnormal erythrocytes decreased, and the fraction of biconcave discs increased. Apparently CH 2Cl 2 is absorbed by the cell membrane and preferentially lyses sickled cells and other abnormal cells. Part of the decrease in NMR relaxation rate with increased CH 2Cl 2 pressure is due to a larger fraction of discs, but an additional factor probably arises from CH 2Cl 2 inhibition of hemoglobin S gelation.
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