Abstract
To investigate the effects of diallyl trisulfide (DT) on apoptosis of cisplatin (DDP)-resistant human epithelial ovarian cancer SKOV-3 cells (SKOV-3/DDP), and the role of p53 upregulated modulator of apoptosis (PUMA). SKOV-3/DDP cells were randomly divided into control, DT, DPP and DPP+DT groups, which were treated with DT or combined DT and DDP. All cells were incubated for 48 h. and apoptosis rates were assessed by flow cytometry. mRNA and protein expression of PUMA, Bax and Bcl-2 was determined by RT-PCR and Western blot assays, respectively. Compared with control group, the apoptosis rates of SKOV-3/DDP cells in DT groups were obviously increased, with dose-dependence (P < 0.05), the mRNA and protein expressions of PUMA, Bax also being up-regulated (P < 0.05), while those of Bcl-2 were down-regulated (P < 0.05). Compared with DT groups, the apoptosis rate in the DDP+DT group was significantly increased (P < 0.05). After knockdown of PUMA with specific siRNA, the apoptosis rate of SKOV-3/DDP cells was obviously decreased (P < 0.05). DT can promote the apoptosis of SKOV-3/DDP cells with PUMA playing a critical role.
Highlights
Ovarian cancer is one of the most common malignant tumors of the female productive tract
Effect of diallyl trisulfide (DT) on apoptosis of SKOV-3/DDP cells Effect of DT on apoptosis of SKOV-3/DDP cells in different groups were shown in Figure 1A and B
Effects of specific p53 upregulated modulator of apoptosis (PUMA) siRNA on SKOV-3/DDP cell apoptosis induced by DT
Summary
Ovarian cancer is one of the most common malignant tumors of the female productive tract. Drug resistance is a major reason to cause reoccurrence of ovarian cancer and treatment failure. It is necessary to seek anti-drug resistance, nontoxic and effective drugs to treat ovarian cancer. Some studies have revealed that, DT can induce the cell apoptosis of many tumors such as lung cancer, breast cancer, colon cancer and skin cancer (Fleischauer and Arab, 2001; Mao et al, 2010; Altonsy and Andrews, 2011; Jeong et al, 2011; Tsubura et al, 2011; Li et al, 2012). Cisplatin (DDP)-resistant human epithelial ovarian cancer SKOV-3 cells (SKOV-3/DDP) were treated with DT or combined DT and DDP.
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