Abstract
Purpose: To investigate the effect of diagnostic ultrasound on corneal apoptosis in rats.Methods: 24 male rats were randomly divided into 4 groups: control group, 10, 20 and 30 min group. The eyeballs of rats were irradiated continuously for different time lengths by Siemens ACUSON S2000 color Doppler ultrasound diagnostic instrument. 24 hours later, the animals were killed and the corneas were taken for Tunel apoptosis detection. The apoptosis rates of corneal epithelial cells, stromal cells and endothelial cells were calculated.Results: Apoptotic cells were detected in corneal epithelial cells, stromal cells and endothelial cells of normal rats. There was no significant difference between the 10 min group and the control group (P>0.05). The apoptosis rate of 20 min and 30 min groups was significantly higher than that of the control group. With the extension of irradiation time, the apoptosis rate of corneal epithelial cells, stromal cells and endothelial cells increased.Conclusion: 20 min of rat eyeball irradiated by diagnostic ultrasound can increase the apoptosis of corneal cells, and the apoptosis is aggravated with the prolongation of ultrasound irradiation time.
 Keywords: Cornea; Ultrasonography; Apoptosis; Epithelial cells
Highlights
Ultrasonic diagnosis has been used in China for many years, and the field of application and the level of diagnostic technology have been continuously improved with the development of science and technology
According to the statistics of the results of TUNEL staining, we found that there were normal apoptotic cells in normal rat corneal epithelial cells, corneal stromal cells and corneal endothelial cells to some extent
There was a significant difference in the number of apoptotic cells in rat corneal epithelial cells at different irradiation time lengths
Summary
Ultrasonic diagnosis has been used in China for many years, and the field of application and the level of diagnostic technology have been continuously improved with the development of science and technology. The main principle of ultrasonic diagnosis is to observe different ultrasonic reflections from different tissue structures of the human body. Observe and analyze by transforming abstract reflected waves into concrete images [1,2]. Some studies have shown that the clarity and resolution of B-ultrasound results need to be improved. The moving probe and dynamic human tissue, as well as the poor conduction of ultrasound in the air, make it difficult to guarantee good image results. Poor image results bring some difficulties for clinical diagnosis [3]. Color Doppler ultrasound is based on the existing black-and-white Bultrasound plus color Doppler, which can observe
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