Abstract
Objective To study the effect of diaethylstilbestrol on the transabdomial phase of testicular descent in the rat.Methods Fifty E 13.5(embryonic day 13.5)pregnant female SD rats were randomlv divided into five groups that received a subcutaneous injection of DMSO,2.5、5、10、20 mg/kg DES respectively.Male offspring were killed at E 19.5,and then fetal mortality,the degree of transabdominal testicular ascent(DTA)was determined by a stereomicroscope.The tuRNA expressions of INSL3、SF-1 in the testis and HOXA10 in the gubernaculums were determined by RT-PCR.The expression of INSL3 protein was determined by western blot.Results Higher fetal mortality,larger DTA were induced by DES dose-dependently(P＜0.01).DES down-regulated the expression of INSL-3 and SF-1 in a dose-effect manner(P＜0.01).Compared with the DMSO group,the expression of HOXA10 mRNA in the group of 2.5 mg/kg was not significant difference(P＞0.05),the expression of HOXA10 mRNA in other groups were down-regulated statistically(P＜0.05).Conclusions DES inhibited transabdominal testicular descent dose-dependently via dose-dependent down-regulating the expression of INSL3,which was induced by dose-dependent down-regulating the expression of SF1.HOXA10 may play no role in low-dosage DES induced abdominal cryptorchidism,but down-regulated HOXA10 mRNA was involved in high-dosage DES induced ones. Key words: Cryptorchidism; Diaethylstilbestrol; Rats
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