Effect of dexmedetomidine on concentrations of hydrogen sulfide, neuron-specific enolase and S100β after cardiopulmonary resuscitation in rats

Abstract

Objective To study the effect of dexmedetomidine on concentrations of hydrogen sulfide (H2S),neuron-specific enolase (NSE) and S100β after cardio-pulmonary resuscitation (CPR) in rats.Methods Sixty SD rats were devided into three groups:sham group (group A),conventional resuscitation group (group B) and dexmedetomidine group (group C) (n =20 each).Blood gas analysis before was compared before asphyxia and after resuscitation among 3 groups.The neurologic deficit scoring (NDS) and the concentrations of H2S,NSE and S100β were compared also.Results After resuscitation,the PaO2 in groups B and C [(45.48 ±5.98) and (48.26 ±6.93) mmHg (1 mmHg =0.133 kPa)] was decreased as compared with before asphyxia [(92.15 ±2.87) and (91.89 ±2.76) mmHg],and PaCO2 [(57.32 ±7.15) and (53.68 ±3.58) mmHg] increased as compared with before asphyxia [(32.98 ± 2.14) and (32.70 ±3.32) mmHg,P ＜0.05].There was significant difference in NDS concentration between groups B and C at different time points (P ＜ 0.05).At 24,48 and 72 h after CPR,there was significant difference in NDS concentration between groups B and C (45.97 ± 5.78,41.32 ±4.79,40.56 ±4.43 and 32.25 ± 4.22,25.82 ± 3.98,17.96 ± 2.79,P ＜ 0.05).There was significant difference in the concentrations of H2S,NSE and S100β between groups B and C at different time points (P ＜ 0.05).At 4,12,24,48 and 72 h after CPR,there was statistically significant difference in H2S and NSE concentrations between groups B and C (P ＜0.05) ; at 12,24,48 and 72 h after CPR,there was significant difference in S100β concentration between groups B and C (P ＜ 0.05).Conclusion Dexmedetomidine can increase the concentrations of H2S as well as reduce the concentrations of NSE and S100β in serum,and it has protective effects on brain of rats to a certain extent. Key words: Dexmedetomidine; Cardiopulmonary resuscitation; Hypoxic ischemic brain damage