Effect of deferoxamine in treatment of chronic hydrocephalus after intravetricular hemorrhage in rats

Abstract

Objective To discuss the effect of deferoxamine in the treatment of chronic hydrocephalus （CH） after intraventricular hemorrhage （IVH） in rats. Methods A total of 184 female Sprague Dawley rats were randomly divided into normal saline group （ NS group）, intracerebroventricular blood infusion group （Group A ） and deferoxamine plus intracerebroventricular blood infusion group （ Group B）. The rat CH models were made by infusing autologous whole blood （ 130 μl） into the right lateral cerebral ventricle. The escape latency time was detected by Morris water maze at days 14 and 28.The rats were sacrificed 1,3, 7, 14, 28 days later, the transverse diameter of the lateral ventricle on the coronal slice of rat brain 0.4 mm posterior to the bregma was measured for evaluation of hydrocephalus and the index of ventricle. Pathological changes of the brain were observed. Results No CH was found in the NS group. The incidence of CH in the Group A was 73% （11/15）, which was significantly higher than 27% （4/15） in Group B at day 28. The escape latency time in the NS group was （8.21 ± 2.00） s,which was significantly shorter than （ 16.36 ±5.93 ） s of the Group A （ P 〈0.01 ）. There was no significant difference in the escape latency time between the NS group and Group B [（11.38 ± 2.57 ）s] （P 〉0.05 ）. The index of ventricle in the NS group was 1.05 ± 0.41, which was smaller than 4.53 ± 1.70 in Group A and 2.77 ± 1.53 in Group B （P 〈 0.05 ）. There were no obvious pathological changes in the NS group at different time points. There found edema of white matter surrounding the ventricle, twist and engorged callosum, proliferation of rhagiocrine cell as well as hemosiderin deposit in Group A. However, no hemosiderin deposit was found in Group B. Conclusions The content of iron in the cerebrospinal fluid may be correlated with hydrocephalus after IVH. Preventive use of deferoxamine can reduce incidence of hydrocephalus after ICH. Key words: Cerebral hemorrhage ; Hydrocephalus ; Deferoxamine