Abstract
The influence of decrease of water absorption rate on the transpiration rate and the development of water saturation deficit (WSD) was studied on the leaf segments of kale. Solutions of polyethyleneglycol (0-25m, 0-50m, O-75m and 1-00m) and mannitol (0-50m) were used as osmotic agents. The rate of water absorption decreased to zero when the concentration of polyethyleneglycol was 0-25m. At a concentration higher than 0-50m, water from the tissue diluted the external solution. The transpiration rate of samples affected by polyethyleneglycol or mannitol was only a little lower than that of control samples. WSD was noticeably increased only in the absorbing part of segment; in the adjoining, transporting part of segment WSD was practically the same in all variants. WSD in the transpiring part was slightly increased only in some cases. Due to decrease of osmotic potential of the external solution also the gradient of water potential changed. In the case of absorption from pure water, water potential gradually decreased from the absorbing to the transpiring part. Under the influence of polyethyleneglycol or mannitol solution the highest water potential was in the transporting part and from this point water potential decreased to both opposite sides.
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