Effect of decoction simplicia pulutan (Urena lobata L.) leaves against the number of follicles strains Balb-C mice

Abstract

Pulutan (Urena lobata L.) is a plant that is used as traditional medicine. The compound in pulutan leaves consists of alkaloids, tannins, triterpenoids, flavonoids, saponins and steroids that work as antifertility agents. The purpose of this study was to determine the effect of pulutan leaves (Urena lobata L.) simplicia to the number of follicles of mice (Mus musculus) Balb-C strain. This study with six treatments giving pulutan leaf simplicia decoction, namely 0%, 5%, 7.5%, 10%, 12.5% and 15%, respectively the treatment repeated four times. Giving treatment for 15 days begins during the metestrus phase. The data analyzed by one way ANOVA, and if they affected, it continue with Duncan test with P <0.05. The results showed the concentration of decoction simplicia pulutan leaves 7,5% began to reduce the number of multilaminar primary and de Graaf follicles and began to increase the number of atresia follicles, whereas at the concentration 10% began to reduce the number of monolaminar primary and secondary follicles. This study concludes that pulutan leaf simplicia decoction can reduce the number of follicles in monolaminar primary, multilaminar primary, secondary follicles, de Graaf follicles and increase the number of atresia follicles.