Effect of Dayak Onion (Eleutherine bulbosa (Mill.) Urb) on Uterine wall and Lipid Profiles of Ovariectomized Rat

Amalia Purnamasari,Anton Bahtiar

doi:10.3844/ojbsci.2018.1.6

Abstract

Menopause is a physiologic condition, in which women no longer experience menstruation. Decreased estrogen during and after menopause causes structural, physiological and biochemical changes that alter the health of women in general, both in organs and non-reproductive organs. Dayak onion contains eleuterinol, an naphtoqunone derivative that can be as SERM. Therefore, we evaluated the effects of dayak onions on lipid profiles and uterine wall. This study used female white rats of Sprague-Dawley. All groups, except the sham group, is ovariectomized to obtain the conditions of hypoestrogen. Rats were divided into 6 groups. SHAM (CMC Na 0,5%), OVX (CMC Na 0,5%), TAM (tamoxifen), DOE dose 1 (dayak onion extract 8 mg/200), DOE dose 2 (dayak onion extract 12 mg/200 g) and DOE dose 3 (dayak onion extract 18 mg/200 g). Mouse model of ovariectomy given 3 doses of extract, from giving each extract it was seen that the weight of uterine DOE dose 1, DOE dose 2 and DOE dose 3 respectively 55,24, 53,38 and 61,85 mg had lower uterine weight when compared with Positive control given tamoxifen 72.2 mg. The effects of onion on uterine weight reduction are greater than that of tamoxifen on the animal model menopause.

Highlights

The menopause is defined as the permanent cessation of menstruation resulting from the loss of ovarian follicular activity
Selective Estrogen Receptor Modulators (SERMs) are a class of compounds that interact with Estrogen Receptors (ER) in target organs as both agonists and antagonists
We have evaluated naphtoquinone compound by TLC as a core of eleutherinol Fig. 1

Summary

Introduction

The menopause is defined as the permanent cessation of menstruation resulting from the loss of ovarian follicular activity. Oestrogen and progesterone receptors are found throughout the urogenital tract and are sensitive to any hormonal changes that occur around the menopause. Hormone Replacement Therapy (HRT, a combination of estrogen and progestin) or Estrogen Replacement Therapy (ERT) has been used for many years as a gold standard for treating menopausal symptoms (Gennari et al, 2010). There are several alternatives to HRT, one of which is Selective Estrogen Receptor Modulators (SERMs). SERMs are a class of compounds that interact with Estrogen Receptors (ER) in target organs as both agonists and antagonists. SERMs compounds have no estrogen steroid structure, but have tertiary structures that can bind to ERα and/or ERβ (Gennari et al, 2010)

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